Highly sensitive and specific graphene/TiO2 impedimetric immunosensor based on plant-derived tetravalent envelope glycoprotein domain III (EDIII) probe antigen for dengue diagnosis

This study reports on the development of a novel impedimetric immunosensor design using plant-derived antigenic glycoprotein for detection dengue virus (DENV) IgG antibodies. The electrochemical platform was constructed screen-printed carbon electrode (SPCE) modified with graphene/titanium dioxide (G/TiO2) nanocomposite to improve in terms performance and specific surface area. A envelope domain III (EDIII) protein used as probe this immunosensing strategy. Under optimised sensing conditions, demonstrated high sensitivity towards DENV wide linear working range (62.5–2000 ng/mL), limit 2.81 ng/mL. showed specificity discriminating against antibodies other infectious disease, including closely related Zika (ZIKV). reliability serological diagnosis verified by challenging serum samples, compared conventional enzyme-linked immunosorbent assay (ELISA). As shown its remarkable throughout study, devised is proposed reliable practical diagnostic tool realistic applications.